Incorporation of 3H-leucine, 3H-lysine, and 3H-tryptophan during the cell cycle of Chinese hamster ovary cells: a flow cytometric analysis.
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منابع مشابه
Differences in the efficiency and pattern of incorporation of [3H]leucine and [3H]proline into proteins of adult cat brain.
Previous EM autoradiographic studies have shown that injection of [3H]proline ([3H]Pro) into the cat dorsal column nuclei (DCN) results in heavy labeling of macroglia but negligible labeling of DCN neurons. [3H]Leucine ([3H]Leu), in contrast, was extensively incorporated into both neurons and glia. We now report preliminary assessment of differences in molecular labeling patterns produced by th...
متن کاملComparison of flow cytometric analysis and [3H]thymidine incorporation for measurement of the effects of drug toxicity on lymphocyte stimulation.
Human mononuclear cells were exposed to three antiviral agents, stimulated with phytohemagglutinin, and assayed for DNA synthesis with [3H]thymidine uptake and flow cytometric analysis. Cytosine-arabinoside demonstrated inhibition of blastogenic reactivity by both [3H]thymidine uptake and flow cytometric analysis, whereas acyclovir showed no significant suppression. In contrast, (E)-5-(2-bromov...
متن کاملA concanavalin A-resistant Chinese hamster ovary cell line is deficient in the synthesis of [3H]glucosyl oligosaccharide-lipid.
In this report we present an initial determination of the biochemical defect present in a Chinese hamster ovary cell line selected for resistance to concanavalin A. Membranes of this mutant, B211, incorporated at least 10-fold less mannose from GDP-[14C]mannose into oligosaccharide-lipid than membranes of the wild type. In the presence of dolichol phosphate, membranes of the mutant and wild typ...
متن کاملIncorporation of [3H]thymidine in mouse spleen.
Mice were given 4 injections of [H]thymidine at 2-hourly intervals and killed at times varying from 1 h to 12 days after the last injection. Autoradiographs of spleen, oesophagus and heart were prepared by the stripping-film technique and exposed for 21, 189 and 367 days. Groups of lymphoid cells surrounding trabeculae were heavily labelled, the decline in grain count suggesting division of the...
متن کاملKinetics of Synthesis and Secretion of [3h]leucine-, [3h]galactose- and [3h]mannose-labelled Myeloma Protein by Two Plasma-cell Tumours by Fritz Melchers
The kinetics of incorporation of leucine, galactose and mannose into intracellular and secreted myeloma protein, MOPC 21 IgG, and MOPC 46 K-type light chain, by cell suspensions of two myeloma plasma-cell tumours, MOPC 21 and MOPC 46, were similar. Radioactive galactose was incorporated to over 90% into galactose residues ofintracellular and secreted protein, mannose to over 90% into glucosamin...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1980
ISSN: 0022-1554,1551-5044
DOI: 10.1177/28.6.6771322